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. 2021 Jan 19;4:82. doi: 10.1038/s42003-020-01606-9

Fig. 6. Transcriptional and translational expression patterns of osteogenic genes in regenerating bone ex vivo.

Fig. 6

Osteogenic related gene expression of ALP, COL1, OCN, and RunX2 were analyzed using qRT-PCR at 4 wks (a–d) and 12 wks (j–m) post-implantation. Protein expression of ALP, COL1, OCN, and RunX2 were analyzed using Western blot analysis in untreated and treated groups at 4 wks (e–i) and 12 wks (n–r) post-treatment. Dollar ($) indicate difference between acellular collagen scaffold group and treatment groups. Hash (#) indicate difference between non-transfection and transfection groups. Asterisk (*) indicate difference among different modRNA transfection groups. Results for qRT-PCR are normalized to the housekeeping gene (rat GAPDH) and to the empty group, and for western blot, the housekeeping protein is rat β-actin. Values are shown as mean ± SD, $/#/*p < 0.05, $$/##/**p < 0.01, $$$/###/***p < 0.001. N = 6, One-way ANOVA followed by Tukey’s multiple comparison.