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. 2021 Jan 12;16(1):20–28. doi: 10.1016/j.stemcr.2020.11.017

Figure 1.

Figure 1

Distinct Protein Synthesis Rates in Fetal and Adult Hematopoietic Stem and Progenitor Cells

(A and B) Protein synthesis in hematopoietic stem and progenitor cells relative to unfractionated cells in (A) young adult BM and (B) E15.5 fetal liver. Data are shown for unfractionated BM cells, liver cells, CD150+CD48−LSK HSCs, CD127−Lineage−SCA1−CD117+ myeloid progenitors (MyP), CD71+TER119+ erythroid progenitors (E), IgM−B220+ B lineage progenitors (B), GR1+ cells (GR).

(C and D) Relative protein synthesis in young adult BM and fetal liver HSCs normalized to (C) unfractionated cells or (D) GR1+ cells.

(E) Relative protein synthesis in young adult BM and fetal liver restricted hematopoietic progenitor cells normalized to GR1+ cells.

All data represent mean ± SD. N = 7 adult mice and 11 embryos. Statistical significance was assessed relative to HSCs using a repeated-measures one-way ANOVA followed by Dunnett's multiple comparisons test (A and B), or using a two-tailed Student's t test to compare differences between fetal and adult cells (C–E); ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.