Figure 2.

Dynamic Changes in Protein Synthesis during Erythroid Differentiation Are Distinct in Adult Bone Marrow and Fetal Liver

(A) Representative flow cytometry plot showing gating strategy for R1-R5 erythroid lineage cells in young adult BM.

(B) Representative histograms showing OP-Puro incorporation in R1-R4 erythroid progenitors in young adult BM.

(C) Relative protein synthesis based on OP-Puro incorporation in young adult R1-R4 erythroid progenitors relative to unfractionated BM cells in vivo. Statistical differences are summarized in the adjacent table.

(D) Representative flow cytometry plot showing gating strategy for R1-R5 erythroid lineage cells in E15.5 fetal liver.

(E) Representative histograms showing OP-Puro incorporation in R1-R4 erythroid progenitors in E15.5 fetal liver.

(F) Relative protein synthesis based on OP-Puro incorporation in E15.5 fetal liver (FL) R1-R4 erythroid progenitors relative to unfractionated liver cells in vivo. Statistical differences are summarized in the adjacent table.

(G and H) Relative protein synthesis in young adult BM and E15.5 fetal liver R1-R4 erythroid progenitors normalized to (G) unfractionated cells or (H) GR1⁺ cells. All data represent mean ± SD. N = 7 adult mice and 11 embryos. Statistical significance was assessed using a repeated-measures one-way ANOVA followed by Tukey's multiple comparisons test (C and F), or using a two-tailed Student's t test to compare differences between fetal and adult cells (G and H); ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.