Figure 4.

The Rpl24^Bst/+ Mutation Impairs Fetal But Not Adult Erythroid Lineage Cells

(A and B) Relative protein synthesis in Rpl24^Bst/+ (Bst/+) or control (+/+) erythroid progenitor cells in (A) young adult BM (N = 4 mice/genotype) and (B) E15.5 fetal liver (N = 12–13 embryos/genotype) in vivo.

(C) Frequency and (D) absolute number of erythroid progenitors in Rpl24^Bst/+ (Bst/+) or control (+/+) young adult BM (1 femur +1 tibia/mouse; N = 3–4 mice/genotype).

(E) Frequency and (F) absolute number of erythroid progenitors in Rpl24^Bst/+ (Bst/+) or control (+/+) E15.5 fetal liver (FL; N = 10 embryos/genotype).

(G) Number of TER119⁺ cells in Rpl24^Bst/+ (Bst/+) or control (+/+) E15.5 fetal liver (N = 10 embryos/genotype).

(H) Frequency of erythroid progenitors that are Annexin V⁺ in Rpl24^Bst/+ (Bst/+) or control (+/+) E15.5 fetal liver (N = 7 embryos/genotype).

(I) Frequency of Rpl24^Bst/+ (Bst/+) or control (+/+) fetal liver erythroid progenitors that incorporated EdU after a 1-h pulse in vivo (N = 3–5 embryos/genotype).

Data represent mean ± SD. Statistical significance was assessed using a two-tailed Student's t test; ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.