Table 1.

Characteristics of primers used for detection of mollicutes investigated in this study

Mycoplasma species	Primer designation	Target gene	Gene locus	Product	Amplicon size (bp)	Sequence (5′-3′)	References
M. hominis	Mhp120’F Mhp120’R	p120’	MHO_RS02125	DUF885 family protein	190	GTTTTAGAACGTAAAATTCTA CATCAGCAGGATTGTTTAAAGCT	This studya
	Mh16SF Mh16SR	rrnA16S	MHO_RS01540	16S ribosomal RNA	164	GGTAATGGCCCACCAAGACTAT GAAGACCTTCATCGTGCACGCTG	This studya
M. genitalium	MgPa335F MgPa432R	mgpA	MG_RS01075	Adhesin protein	78	GAGAAATACCTTGATGGTCAGCAA GTTAATATCATATAAAGCTCTACCGTTGTTATC	[43]
	Mg16SF MG16SR	rrnA16S	MG_RS00775	16S ribosomal RNA	195	TAGCTAATACCGCATAAGAACTTT CTCCCGTAGGAGTATGGGCCGTGT	This studya
M. fermentans	RW 005 RW 004	IS1630I	MBIO_RS03870	IS30-like element	205	GGTTATTCGATTTCTAAATCGCCT GGACTATTGTCTAAACAATTTCCC	[44]
	Mf16SF Mf16SR	rrnA16S	MBIO_RS4090	16S ribosomal RNA	186	TTGACGGTACCTTATTAGAAA TCTACACAACTCTAGCCTG	This studya
U. urealyticum	UUureF UUureR	ureB	UUR8_RS02385	Urease subunit beta	100	GATCACATTTCCACTTATTTGAAACA AAACGACGTCCATAAGCAACTTTA	[45]
	Uu16SF Uu16SR	rrnA16S	UUR8_RS00640	16S ribosomal RNA	181	TGCCTGGGTAGTACATTCGCAAGAA GACAACCATGCACCACCTGTC	This studya
U. parvum	UPureF UPureF	ureB	UPA3_RS02275	Urease subunit beta	99	GATCACATTTTCACTTGTTTG AAGTG AACGTCGTCCATAAGCAACTTTG	[45]
	Up16SF Up16SF	rrnA16S	UPA3_RS00595	16S ribosomal RNA	183	CTTTTATATGGGAAGAAACGCT GCATCTAGATTTAATACC	This studya
Human DNA	HmitoDNAF HmitoDNAR	MT-CO3	YP_003024032.1	Cytochrome c oxidase subunit III	208	GGATAATCCTATTTATTACCTCAGAAG AGACTATGGTGAGCTCAGGTGATTGAT	This studya

^aPrimers for each gene were designed based on sequences available in GenBank