Concentration (ng/mL) of 7β-hydroxy and 7-oxo C24 and C27 acids in SLOS (n = 10) and control (n = 24) plasma. (A) 3βH,7O-CA. (B) 3βH,7O-Δ5-BA. (C) 3β,7β-diHCA. (D) 3β,7β,24-triHCA. (E) 3β,7β,25-triHCA. (F) 3β,7β-diH-Δ5-BA. See Supplemental Figure S2 for relevant chromatograms. Concentrations determined and statistical comparisons were as described in the caption to Fig. 2.