Figure 5.
Graft-infiltrating, recipient-derived T cells take on a TRM cell phenotype over time, with CD103+ and CD103− CD8+ T cells displaying distinct phenotypes
(A) Fluorescence microscopy chip cytometry image of small intestinal transplant mucosa from a single subject 3 months after transplant. The donor was HLA-A3+, and the recipient was HLA-A3−. False-color fluorescence imaging for cytokeratin (gray), CD3 (purple), CD8 (red), CD103 (blue), and HLA-A3 (green). Donor-derived CD8+ CD103+ and CD103− T cells are indicated by white and yellow arrows, respectively.
(B) Phenotypic analysis of CD8+ T cell populations in healthy small intestinal epithelium and lamina propria. Proportion of positive cells (CD161) or MFI (CD7, CD127, β2-integrin, and KLRG1) of intraepithelial lymphocyte (IEL) or lamina propria lymphocyte (LPL) CD8+ T cells, categorized by CD69 and CD103 expression, in small intestinal biopsies from healthy control subjects (n = 4). Mean percentage or MFI represented by bars. Connecting lines represent populations from the same subject.
(C) Fluorescence microscopy chip cytometry of representative CD103− (cells 1 and 2) and CD103+ (cells 3 and 4) donor-derived CD8+ T cells, showing the expression of CD18 (β2-integrin), KLRG1, and granzyme K.
Statistical analysis performed with one-way ANOVA with Tukey’s multiple-comparison test. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.