Figure 7.

CD103⁺ and CD103⁻ CD4⁺ T cells display analogous phenotypic and functional differences to their CD8⁺ counterparts

(A) MFI (CD161, CD7, CD127, β2-integrin, granzyme K, and KLRG1) or percentage positive (Ki-67) of CD4⁺ T cells, categorized by CD69 and CD103 expression, in small intestinal biopsies from healthy control subjects (n = 10). MFI represented by bars. Connecting lines represent populations from the same subject. Blue, CD69⁻CD103⁻ cells; red, CD69⁺CD103⁻ cells; black, CD69⁺CD103⁺ cells.

(B) MFI of β2-integrin on recipient-derived CD4⁺ T cells, categorized by CD69 and CD103 expression and time after transplant, in intestinal transplant grafts (n = 21; 12 subjects). MFI represented by bars.

(C–H) Cytokine production by small intestinal CD4⁺ T cells. Representative histograms of expression, and group summaries of proportion of CD4⁺ T cells expressing IL-17A (C), TNF-α (D), IFN-γ (E), IL-2 (F), CCL4 (G), and IL-10 (H) after 4 h stimulation with PMA and ionomycin in the presence of brefeldin A and monensin, categorized by CD69 and CD103 expression, in small intestinal biopsies from healthy control subjects (n = 10).

(I) Mean proportion of CD4⁺ T cells expressing 0, 1, 2, 3, 4, 5, or 6 of the cytokines or chemokines IL-17A, TNF-α, IFN-γ, CCL4, IL-2, and IL-10, categorized by CD69 and CD103 co-expression, from small intestinal biopsies from healthy control subjects (n = 10).

Statistical analysis performed with one-way ANOVA with Tukey’s multiple-comparison test. ^∗p ≤ 0.05, ^∗∗p ≤ 0.01, ^∗∗∗p ≤ 0.001.