Figure 2.
PCR analysis of β-lactamase gene levels and transcript levels for Patient 4 isolates (4A–D). (a) TaqMan qPCR of genomic DNA results collected in triplicate on two separate days (n = 6) for either blaOXA-1 or blaCTX-M-15 relative to the endogenous control gene rpsL. Data shown are individual data points with mean ± SD superimposed. (b) blaOXA-1 transcript level relative to endogenous control gene rpsL. RNA was collected from mid-exponential phase in triplicate on two separate days (n = 6). Data shown are mean ± SD. (c) Similar analysis to (b) except that data are for blaCTX-M-15. P values refer to measurements in the serial isolates relative to the initial isolate using the Kruskal–Wallis test.