KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-Mouse CD45.1 (PB conjugated, clone A20) | Biolegend | Cat#110721; RRID: AB_492867 |
| Anti-Mouse CD45.1 (APC conjugated, clone A20) | eBioscience | Cat#17–0453-81; RRID: AB_469397 |
| Anti-Mouse CD45.2 (BV 605 conjugated, clone 104) | Biolegend | Cat#109841; RRID: AB_2563485 |
| Anti-Mouse CD45.2 (PE conjugated, clone A20) | eBioscience | Cat#12–0453-81; RRID: AB_465674 |
| Anti-Mouse c-kit/CD117 (APC-Cy7 conjugated, clone 2B8) | eBioscience | Cat#25–1171-82; RRID: AB_469644 |
| Anti-Mouse CD4 (PE-Cy5 conjugated, clone GK 1.5) | eBioscience | Cat#15–0041-82; RRID: AB_468695 |
| Anti-Mouse CD4 (FITC conjugated, clone GK1.5) | eBioscience | Cat#11–0041-82; RRID: AB_464892 |
| Anti-Mouse CDS (PE-Cy5 conjugated, clone 53–6.7) | eBioscience | Cat#15–0081-82; RRID: AB_468706 |
| Anti-Mouse CDS (FITC conjugated, clone 53–6.7) | eBioscience | Cat#11 −0081 −82; RRID: AB_464915 |
| Anti-Mouse CD45R/B220 (PE-Cy5 conjugated, clone RA3–682) | eBioscience | Cat#15–0452-82; RRID: AB_468755 |
| Anti-Mouse CD45R/B220 (PE-Cy7 conjugated, clone RA3–6B2) | eBioscience | Cat#25–0452-82; RRID: AB_469627 |
| Anti- Mouse/Human CD45R/B220 (FITC conjugated, clone RA3–6B2) | eBioscience | Cat#11–0452-82; RRID: AB_465054 |
| Anti-Mouse Ly-6G/Gr1 (PE-Cy5 conjugated, clone RB6–8C5) | eBioscience | Cat#15–5931-82; RRID: AB_468813 |
| Anti-Mouse Ly-6G/Gr1 (PE-Cy7 conjugated, clone RB6–8C5) | eBioscience | Cat#25–5931-82; RRID: AB_469663 |
| Anti-Mouse CD11b/Mac1 (PE-Cy5 conjugated, clone M1/70) | eBioscience | Cat#15–0112-82; RRID: AB_468714 |
| Anti-Mouse CD11b/Mac1 (PE-Cy7 conjugated, M1/70) | eBioscience | Cat#25–0112-81; RRID: AB_469587 |
| Anti-Mouse Ter119 (PE-Cy5 conjugated, clone M1/70) | eBioscience | Cat#15–5921-82; RRID: AB_468810 |
| Anti-Mouse CD150/SLAM (PE-Cy7 conjugated, clone TC15–12F12.2) | Biolegend | Cat#115914; RRID: AB_439797 |
| Anti-Mouse CD48 (APC conjugated, clone HM48–1) | eBioscience | Cat#17–0481-82; RRID: AB_469408 |
| Anti-Mouse CD34 (FITC conjugated, clone RAM 34) | eBioscience | Cat#11–0341-82; RRID: AB_465021 |
| Anti-Mouse Flk2/Flt3/CD135 (PE conjugated, clone A2F10) | eBioscience | Cat#12–1351-82; RRID: AB_465859 |
| Anti-Mouse Annexin V (PE conjugated) | BD PharMingen | Cat#556422; RRID: AB_2108590 |
| Anti-Human CD34 (PB conjugated, clone RAM 34) | eBioscience | Cat# 48–0341-82; RRID: AB_2043837 |
| DAPI | Life technologies | Cat#D1306; RRID: AB_2629482 |
| Anti-E-selectin Rat monoclonal anti body (clone 9A9) | Bio X cell | Cat# BE0294; RRID: AB_2687816 |
| Purified Rat lgG1 lambda isotype control (cloneA110–1) | BD | Cat#553993; RRID: AB_395190 |
| Anti-Mouse CD184 (BV605 conjugated, clone 2B11) | BD | Cat#740378; RRID: AB_2740109 |
| Anti-Mouse lgG2b,k (BV605 conjugated, clone R35–38) | BD | Cat#563145; RRID: N/A |
| Anti-Human CD317 (PE conjugated, clone 26F8) | eBioscience | Cat#12–3179-42; RRID: ABJ0596640 |
| Anti-Mouse CD317(Biotin conjugated, clone eBio927) | eBioscience | Cat#13–3172-82; RRID: AB_763415 |
| Bacterial and Virus Strains | ||
| Mycobacterium avium | N/A | strain SmT2151 |
| Biological Samples | ||
| Human Buffy coat samples | Gulf Coast Regional Blood Center | https://www.gulfcoastconsortia.org |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Mouse IFNy | eBioscience | Cat#BMS326 |
| Recombinant Human IFNy | RD Biosystems | Cat#285-IF-100 |
| Recombinant Human Flt3-Ligand | Preprotech | Cat#300–19 |
| Recombinant Human IL-3 | Preprotech | Cat#2 00–03 |
| Recombinant Human SCF | Preprotech | Cat#300–07 |
| Recombinant Human TPO | Preprotech | Cat#300–18 |
| Recombinant Mouse TGFp | R&D Biosystems | Cat#7346-B2/CF |
| Mouse E-selectin | R&D Biosystems | Cat#575-ES100 |
| Mouse P-selectin | R&D Biosystems | Cat#737-PS-050 |
| Human E-selectin | R&D Biosystems | Cat#724-ES-100 |
| Mouse CD117 Micro Beads | MACS Miltenyi Biotec | Cat#130–091 −224 |
| Human CD34 Micro Beads | MACS Miltenyi Biotec | Cat#130–046-702 |
| CFSE | Life Technologies | C34554 |
| DDAO | Life Technologies | C34553 |
| Recombinant human TNFa protein | Fisher | PHC3016 |
| Tamoxifen | SantaCruz Biotechnology | CAS 10540–29-1 |
| Critical Commercial Assays | ||
| NucleoSpin RNA Plus XS kit | Macherey-Nagel | Cat# 740990 |
| SMARTer® Stranded Total RNA-Seq Kit i/2 - Pico Input Mammalian | Takara | Cat# 634411 |
| RNAqueous Kit | Ambion | Cat#AM1912 |
| Superscript III First-Strand Synthesis Supermix | Invitrogen | Cat#18080051 |
| iTaq Universal SYBR Green Supermix | BioRad | Cat#172–5121 |
| BrdU Flow Kit (FITC conjugated) | BD | Cat# 559619 |
| Deposited Data | ||
| https://data.mendeley.com/datasets/mhc8b4yn9d/1 | N/A | N/A |
| Experimental Models: Organisms/Strains | ||
| CD45.1 mice | Baylor College of Medicine | JAX Stock#0066584 |
| CD45.2 mice | Baylor College of Medicine | Stock#14170 |
| Bst2−/−mice | Jackson Labs | Stock#029892 |
| Ifngr1−/− mice | Jackson Labs | Stock#025545 |
| CXCL12GFP/+ | (Toshiaki et al., 2003) | N/A |
| Krt18-CreER (Tg(KRT18-cre/ERT)23Blpn/J | Dr. Nakada Daisuke, Baylor College of Medicine | N/A |
| B6.Cg-Gt(ROSA)26Sor1m14(CAG”1dToma1o)Hz7 J | Jackson Labs | JAX:007914 |
| Oligonucleotides | ||
| sgRNA: BST2+17403843 - UGC GGUACAGAUGGCAAAUC | Synth ego | N/A |
| sgRNA: BST2–17403817 - GUA CCG CAG AAGAG AAAACC | Synth ego | N/A |
| sgRNA: BST2+17403810 - CGA UUCUCACGCUUAAGACC | Synth ego | N/A |
| sgRNA: BST2+17403763 - CGC A.GCGGAGCUGGAGUCCU | Synth ego | N/A |
| Forward Primer 1: GATCAAG G G AATGTTCAAGCG AAA | Integrated DNA Technologies | N/A |
| Reverse Primer 1: AGGATCT CCTTTGCTCCCAAAATC | Integrated DNA Technologies | N/A |
| Forward Primer 2: CATCCTT CTCACTG G ATTCTCCC | Integrated DNA Technologies | N/A |
| Reverse Primer 2: ATTAAAC CATAAG CTTCAG GACG C | Integrated DNA Technologies | N/A |
| Forward sequencing primer 1: CTGCAGCCTCT CTCTCTAGACTT | Integrated DNA Technologies | N/A |
| Forward sequencing primer 2: CTGCAGCCTCT CTCTCTAGACTTC | Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| Prism | N/A | N/A |
| Flow Jo | N/A | N/A |
| Fiji | N/A | N/A |
| Leica Application Suite software (Version 3.3), Leica Microsystems | N/A | N/A |
| Other | ||
| StemSpan SFEM | StemCell Technologies, Inc. | Cat#9600 |