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Quantitative polymerase chain reaction primer pairs for marker molecule analysis.
| Molecule | Primer sequence (5′-3′) |
|---|---|
| Twist-F | GTCCGCAGTCTTACGAGGAG |
| Twist-R | GCTTGAGGGTCTGAATCGGGCT |
| Slug-F | GCTACCCAATGGCCTCTCTC |
| Slug-R | CTTCAATGGCATGGGGGTCT |
| Snail-F | TCGGAAGCCTAACTACAGCGA |
| Snail-R | AGATGAGCATTGGCAGCGAG |
| β-actin-F | CATGTACGTTGCTATCCAGGC |
| β-actin-R | CTCCTTAATGTCACGCACGAT |
F, forward; R, reverse.
