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. 2021 Jan 8;21(3):193. doi: 10.3892/ol.2021.12454

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Overexpression of OTUD6B-AS1 inhibits the proliferation of HCT116 cells. (A) mRNA expression levels of OTUD6B-AS1 were determined using reverse transcription-quantitative PCR following transfection with OTUD6B-AS overexpression plasmid. The proliferation of HCT116 cells was measured using (B) Cell Counting Kit-8 and (C) colony formation assays. (D) Expression levels of proliferation-related protein Ki67 were detected by immunofluorescence staining. Magnification, ×200. *P<0.05 and ***P<0.001 vs. Oe-NC. OTUD6B-AS1, ovarian tumor domain containing 6B antisense RNA1; NC, negative control; Oe, overexpression; OD, optical density; lncRNA, long non-coding RNA.