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. 2021 Jan 8;21(3):193. doi: 10.3892/ol.2021.12454

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — miR-3171 mimic reverses the inhibitory effect of OTUD6B-AS1 overexpression on the proliferation of HCT116 cells. The proliferation of HCT116 cells was examined using (A) Cell Counting Kit-8 and (B) colony formation assays following co-transfection of OTUD6B-AS1 overexpression plasmid and miR-3171 mimic. (C) Expression of proliferation-related protein Ki67 was detected by immunofluorescence staining. Magnification, ×200. *P<0.05 and ***P<0.001 vs. Oe-NC; ##P<0.01 vs. Oe-OTUD6B-AS1+mimic-NC. OTUD6B-AS1, ovarian tumor domain containing 6B antisense RNA1; NC, negative control; Oe, overexpression; miR-3171, microRNA-3171; OD, optical density.