Fig. 6.
Knockdown of circSDHC inhibits the progression of RCC tumor in vivo. a. Stable circSDHC knockdown 786-O cell line establishment. Relative expression levels of linear SDHC and circSDHC were measured by qRT-PCR. Expression level were normalized to control vector group. b. Weekly mice body weight change in the metastasis experiment. 1 × 106 stable circSDHC knockdown or control 786-O cells were injected via tail vein (n = 8 in each group). c. Representative images of gross and microscopic HE stain of the tumor-infiltrated lung (left). The metastatic foci in each mouse from two groups were counted under microscope and summarized (right). d. The picture of the gross tumors in dissected from subcutaneous xenograft model (left). 5 × 106 stable circSDHC knockdown or control 786-O cells were inoculated subcutaneously into the left side of the body. (n = 8 in each group). Weekly tumor volume change was recorded and showed (right). e. The final tumor weights in the subcutaneous xenograft model. f. HE and IHC staining of the tumors from subcutaneous xenograft model. g. Summary and statistical analysis of CDKN3 and E2F1 IHC scores