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. 2021 Jan 19;21:58. doi: 10.1186/s12935-021-01766-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — IRF4 overexpression promoted the transdifferentiation of Tregs into macrophage-like cells by inhibiting BCL6 expression. Tregs were transfected with LV-IRF4 or LV-ctrl. Normal Tregs served as control. QRT-PCR (a) and WB (b) were performed to assess the gene and protein expression of BCL6 in the modified Tregs. c The interaction between IRF4 and BCL6 promoter was verified by ChIP. Tregs were transfected with LV-IRF4, LV-BCL6 or LV-ctrl. LV-IRF4 and LV-BCL6 were co-transfected into Tregs. Normal Tregs served as control. d The proportions of macrophages in the modified Tregs were detected by flow cytometry. e The phagocytosis assay was performed to assess the phagocytosis of the modified Tregs. The phagocytosis positive cells were marked by arrows. (**P < 0.01, versus LV-ctrl; ^##P < 0.01, versus LV-BCL6)