Fig. 3.

Silencing of BCOR increased cell proliferation, AKT phosphorylation, and IL-2 production. Jurkat cells were transfected with scrambled siRNA or BCOR siRNA oligonucleotides. a After transfection, relative cell proliferation was determined using a cell counting kit (CCK-8) for the indicated time. Data are shown as the mean ± SEM of five independent experiments performed in triplicates (**P < 0.01 compared with cells transfected with scrambled siRNA). b Cell lysates (20 μg) were prepared 48 h after transfection and processed for immunoblotting with the indicated antibodies. Alpha-tubulin was used as the loading control. This result is representative of three independent experiments. The uncropped blots are shown in Supplementary Figure S5 (Additional file 6). c Analysis of the production of IL-2 by ELISA. After transfection, cells were stimulated with PMA and ionomycin. Data are shown as the mean ± SEM of five independent experiments performed in triplicates (**P < 0.01 compared with cells transfected with scrambled siRNA)