Fig. 4.

Circ-FANCA regulated LPS-induced HK2 cell injury by targeting miR-93-5p. a The knockdown efficiency of anti-miR-93-5p was analyzed by qRT-PCR. HK2 cells were divided into 6 groups: Control, LPS, LPS+ si-NC, LPS+ si-circ-FANCA, LPS+ si-circ-FANCA +anti-miR-NC and LPS+ si-circ-FANCA+ anti-miR-93-5p. b The qRT-PCR was performed for miR-93-5p expression detection in the above groups. c–e The measurement of cell viability (c), cell apoptosis (d), cell cycle distribution (e) was carried out via CCK-8 assay or flow cytometry in the above groups. f The release of IL-1β and TNF-α was investigated by ELISA. g, h The activities of SOD and MDA in these cells were determined using the corresponding kits. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001