Fig. 1.

Schematic overview of experimental design. PBMCs from 8 healthy HLA-mismatched donors (tubes on left) were barcoded with MULTI-seq LMOs (black double-helix hybridized to red DNA barcode) and BD single-cell multiplexing kit (SCMK) antibodies (black antibody conjugated to teal DNA barcode). Cells were then strategically pooled to directly assess whether mixing HLA-mismatched PBMCs during scRNA-seq causes alloreactivity