Table 3.
Antigenicity, allergenicity, solubility, and physicochemical property assesment of the primary sequence of multi-epitope-based vaccine construct
| Sl. No | Features | Assessment |
|---|---|---|
| 1 | Antigenicity |
1.2317 (Probable ANTIGEN) by VaxiJen v2.0 0.918291 (Probable ANTIGEN) by ANTIGENPro |
| 2 | Allergenicity |
Probable non-allergen (AllerTOP v.2.0) Probable non-allergen (AllergenFP v.1.0) |
| 3 | Solubility |
0.668 (Soluble) |
| 4 | Number of amino acids | 632 |
| 5 | Molecular weight |
64,366.48 Dalton |
| 6 | Theoretical Isoelectric point (pI) | 5.12 |
| 7 | Total number of atoms | 8920 |
| 8 | Formula | C2764H4392N800O957S7 |
| 9 | Estimated half-life |
30 h (mammalian reticulocytes, in vitro) > 20 h (yeast, in vivo) > 10 h (Escherichia coli, in vivo) |
| 10 | Instability index |
27.70 (Stable) |
| 11 | Aliphatic index | 59.45 |
| 12 | Grand average of hydropathicity (GRAVY) | − 0.646 |
Bold denotes that antigenic score is higher than 0.45 (threshold value)