Table 1.
Inhibitory effects of Qingfei Paidu decoction on major P450s and CES1A (with 3 min or with 33 min pre-incubation) in HLMs.
| Probe reaction | Target enzyme | Time-dependent inhibition IC50 (μg/mL) |
Ratio | |
|---|---|---|---|---|
| Pre-incubation for 3 min | Pre-incubation for 33 min | |||
| Phenacetin O-deethylation | CYP1A | 174.4 ± 7.7 | 143.3 ± 13.0 | 1.21 |
| Coumarin 7-hydroxylation | CYP2A6 | 498.3 ± 50.6 | 484.5 ± 25.3 | 1.03 |
| Paclitaxel 6α-hydroxylation | CYP2C8 | 337.7 ± 43.3 | 355.2 ± 57.9 | 0.95 |
| Diclofenac 4′-hydroxylation | CYP2C9 | 706.8 ± 71.1 | 846.7 ± 92.6 | 0.83 |
| Omeprazole 5-hydroxylation | CYP2C19 | 1244.0 ± 201.3 | 790.3 ± 84.2 | 1.70 |
| Dextromethorphan O-demethylation | CYP2D6 | 1253.0 ± 105.7 | 1991.0 ± 245.5 | 0.63 |
| Chlorzoxazone 6-hydroxylation | CYP2E1 | 469.3 ± 24.3 | 541.5 ± 34.1 | 0.87 |
| Testosterone 6β-hydroxylation | CYP3A | 762.1 ± 65.6 | 324.4 ± 33.6 | 2.35 |
| DME-hydrolysis | CES1A | 1336.0 ± 173.2 | 1436.0 ± 192.2 | 0.93 |