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. 2020 Dec 30;7(1):veaa100. doi: 10.1093/ve/veaa100

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Published by Oxford University Press 2021.

This work is written by US Government employees and is in the public domain in the US.

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Figure 3. — Comparison of virus discovery tools for DNA datasets. Contigs >1,000 nucleotides were analyzed using four virus detection/discovery pipelines. (A) A dataset for human stool enriched for nuclease-resistant DNA in virus-sized particles (SRR6128021). Left panel: Venn diagram displaying the overlap of contigs identified as viruses by the various pipelines. Middle panel: maps showing representative examples of unique calls from each pipeline. Left panel: scatter plots display the length distribution for calls unique to each software package. Rows of plots show contigs that contain both virion structural genes and DNA or RNA replication-associated genes, only one of the two categories of gene, or neither. (B) Similar display of a dataset for Amazon River water samples analyzed with shotgun total DNA sequencing (ERR2338392). (C) Similar display of a dataset for wastewater enriched for nuclease-resistant DNA in virus-sized particles amplified using MDA/RCA (SRR3580070).