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. 2020 Dec 22;64(1):695–710. doi: 10.1021/acs.jmedchem.0c01705

Figure 5.

Figure 5

Comparing the effect of new analogues on STAT1, STAT3, or STAT5 DNA-binding activity in vitro. Nuclear extracts of equal total protein prepared from epidermal growth factor-stimulated NIH3T3/EGFR fibroblasts containing activated STAT1, STAT3, and STAT5 were preincubated with increasing concentrations of the designated compounds for 30 min at room temperature prior to incubating with the radiolabeled hSIE probe that binds STAT1 and STAT3 (upper panel) or the MGFe probe that binds STAT5 (bottom panel) and performing EMSA analysis. Positions of STAT:DNA complexes in gel are labeled; control lanes (0) represent nuclear extracts preincubated with 10% DMSO. Data are representative of two to three independent determinations.