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. 2020 Dec 22;64(1):695–710. doi: 10.1021/acs.jmedchem.0c01705

Figure 6.

Figure 6

Effects of compounds on constitutive STAT3 activation in tumor cells. (A) Nuclear extracts of equal total protein prepared from the human breast cancer, MDA-MB-468 cells untreated (DMSO, 0) or treated with 5 μM of the indicated analogues for 1–3 h were subjected to STAT3 DNA-binding assay using the hSIE probe that binds STAT3, and (B) immunoblotting analysis of whole-cell lysates of equal total protein prepared from (i) and (ii) MDA-MB-231 cells untreated (DMSO, 0) or treated with 1 or 3 μM of 7g for 3–24 h or (iii) MDA-MB-468 cells untreated (DMSO, 0) or treated with 3 μM of 9k for 3–24 h and probing for pY705STAT3, STAT3, or tubulin. Positions of STAT3:DNA complex or proteins in gel are shown; control (0 or Con) lane represents whole-cell lysates or nuclear extracts prepared from 0.05% DMSO-treated cells. Data are representative of two to three independent determinations.