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Inhibition of STAT3 target gene expression in breast cancer cells. Immunoblotting analysis of whole-cell lysates of equal total protein prepared from MDA-MB-231 cells untreated (DMSO, C) or treated with 1 or 3 μM of 7g for 3–24 h and probing for c-Myc Bcl-2, VEGF, survivin, or tubulin. Positions of proteins in gel are shown; control (C) lane represents whole-cell lysates prepared from 0.05% DMSO-treated cells. Data are representative of two to three independent determinations.
