Fig. 3. Characterization of αS aggregation in the presence of kosmotropic salts. (A and B) Aggregation kinetics of 100 μM αS incubated in the presence of different concentrations of NaCl (A) or Na₂SO₄ (B) (concentrations expressed in ionic strength). Three representative replicas are shown from more than 10 measurements with three different protein batches. (C) IR spectra of aggregates formed in 2 M NaCl (green solid line), 3.5 M NaCl (green dotted line), 2 M Na₂SO₄ (brown solid line) and 3 M Na₂SO₄ (brown dotted line) (concentrations expressed in ionic strength). Representative AFM images of aggregates formed in 2 M NaCl (D) and 3.5 M NaCl (E). Scale bar: 200 nm.