Fig. 5. Propensity of αS to aggregate in the absence of an active hydrophobic/hydrophilic interface. (A) Surface tension measurements of αS solutions in PBS alone (blue), with 10% MeOH (pink) or with 35% MeOH (brown); average measurement error 0.82, 1.15 and 2.07 mN m–1 for each solution condition, respectively. (B) Outline of the microfluidic system used for the experiments shown in panels (C) and (D). (C and D) Aggregation kinetics of 100 μM αS in the presence of 15% TFE (C) or 3.5 M NaCl (D) incubated in the microfluidic system (black points). Bars represent standard errors for n = 3–4 measurements per aggregation time point. Solid colored lines show data from standard aggregations in the microplate.
