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Fig. 6. Intermolecular pyrene excimer formation analysis of the different β-sheet αS amyloid aggregates. (A) Pyrene E/M intensity ratio values obtained for the αS aggregates labeled with a 1 : 10 pyrene-αS/unlabeled-αS ratio at different sequence positions formed in PBS with 10% MeOH (A, red), PBS with 35% MeOH (A, shaded red), PBS with 2 M NaCl (B, green) or PBS with 3.5 M NaCl (shaded green), pH 7.4.