Fig. 3.

Western blot analysis of Runt-related transcription factor 2 (RUNX2), osteopontin and alkaline phosphatase (ALP) DPH-induced osteogenic differentiation in DPSCs culture. Four groups were analyzed. Cells treated with: In 4 groups: C, OBM, ODM, DPH (12.5, 50, 100 and 200 μM) as described Methods and materials. The protein levels of alkaline phosphatase (ALP), Runt-related transcription factor 2 (RUNX2) and osteopontin (OSP) were detected by western blot analysis at day 10. β-Actin was used as the internal control. Data are expressed as mean ± SEM. **p< 0.01, ***p< 0.001 vs. control.