Table 2.

TBARS values in Pre-treatment and Post-treatment groups.

Treatment	Cell line	Thymol (µg/ml) t-BHP (µM)	0	50	100	200	400	800
Pre-treatment	MCF-7	0	49.3±0.05	53.7±0.03	44.8±0.01	30.5±0.03	34.8±0.03	32.5±0.02
		50	32.8±0.01	54.03±0.03	47.7±0...6	36.5±0.03	34.5±0.03	32±0.03
	Fibroblast	0	59.2±0.03	39.7±0.01	49.7±0.01	37.5±0.01	43.5±0.01	46.8±0.03
		50	57.5±0.01	79.7±0.06	79.7±0.05	86.2±0.05	79.7±0.02	69.7±0.05
Post-treatment	MCF-7	0	42.9±0.00	53.7±0.03	44.8±0.01	30.5±0.03	34.8±0.03	32.5±0.02
		50	32.8±0.01	31.3±0.03	30.5±0.02	24.3±0.02	28.5±0.01	24.8±0.01
	Fibroblast	0	59.2±0.03	39.7±0.01	49.7±0.01	37.5±0.01	43.5±0.01	46.8±0.03
		50	57.5±0.01	51.8±0.01	55.5±0.00	56.3±0.01	59.03±0.01	61.7±0.07

*In the pre-treatment group, MCF-7 and fibroblast cells were pre-incubated with thymol (0, 50, 100, 200, 400, or 800 µg/ml) for 24 h; then t-BHP was added (0 or 50 μM) for 1 h. In the post-treatment group, MCF-7 and fibroblast cells were first exposed with 50 μM t-BHP for 1 h, then Thymol (0, 50, 100, 200, 400, or 800 µg/ml) was added for 24 h. After 24 h incubation, the culture medium was collected for TBARS assay by ELISA reader. All results were shown mean ± SD of three separated experiments.