Table 1.
Primers ID | Sequence (5' to 3') | Target gene | Size(bp) | Annealing temperature (°C) |
---|---|---|---|---|
slyD-F | GCGAAGGTGAAAATGGCGAA | slyD | 216 | 62 |
slyD-R | GATGATCGTGTCCATGTCCG | |||
MD16S-F | ATCTAATGGCTTAACCATTAAAC | 16SrRNA | 857 | 56.4 |
MD16S-R | GGACGGTAACTAGTTTAGTATT | |||
cadF-F | TAAAAGCGGTGGATTTGGAC | cadF | 218 | 54 |
cadF-R | CAGGACATTTTGCTTGTGGA | |||
rpoA-F | CGAGCTTGCTTTGATGAGTG | rpoA | 121 | 52 |
rpoA-R | AGTTCCCACAGGAAAACCTA | |||
dnaJ-F | GGCAGGGGACAAGTAGGAA | dnaJ | 227 | 52 |
dnaJ-R | CCCCTATTGCCACTTTTGCT |