Table 3.
Sensitivity and specificity of PCR.
| Total Sample | Gene Diagnostic | Result | Person | Percentage | Diagnostic analysis | Chi-square analysis | p value | DF |
|---|---|---|---|---|---|---|---|---|
| 30 samples Patient + 30 samples healthy people | Detection With 16SrRNAgene | 20 positive | 15 Patient | 25% | Sensitivity: 50.00% | 7.375 | 0.006 | 1 |
| 5 healthy | 8.3% | (31.297%-68.703%) Specificity: 83.33% | ||||||
| 40 Negative | 15 Patient | 25% | (65.279%-94.358%) | |||||
| 25 healthy | 41.7% | Positive Predictive Value: 75.00% | ||||||
| (55.529%-87.816%) Negative Predictive Value: | ||||||||
| 62.50%(52.967%-71.153%) Disease prevalence: | ||||||||
| 50.00%(36.806%-63.194%) | ||||||||
| 30 samples Patient + 30 samples healthy people | Detection With cadF gene | 21 positive | 16 Patient | 26.7% | Sensitivity: 53.33% | 8.717 | 0.0032 | 1 |
| 5 healthy | 8.3% | (34.326%-71.658%) Specificity: 83.33% | ||||||
| 39 Negative | 14 Patient | 23.3% | (65.279%-94.358%) | |||||
| 25 healthy | 41.7% | Positive Predictive Value: 76.190% | ||||||
| (57.341%-88.396%) Negative Predictive Value: | ||||||||
| 64.103%(54.119%-72.998%) | ||||||||
| Disease prevalence: 50.00% | ||||||||
| (36.806%-63.194%) | ||||||||
| 30 samples Patient + 30 samples healthy people | Detection With slyDgene | 21 positive | 16 Patient | 26.7% | Sensitivity: 53.33% | 8.717 | 0.0032 | 1 |
| 5 healthy | 8.3% | (34.326%-71.658%) Specificity: 83.33% | ||||||
| 39 Negative | 14 Patient | 23.3% | >(65.279%-94.358%) | |||||
| 25 healthy | 41.7% | Positive Predictive Value: 76.190% | ||||||
| (57.341%-88.396%) Negative | ||||||||
| Predictive Value: 64.103% (54.119%-72.998%) | ||||||||
| Disease prevalence: 50.00% | ||||||||
| (36.806%-63.194%) | ||||||||
| 30 samples Patient + 30 samples healthy people | Detection With rpoA gene | 20 positive | 15 Patient | 25% | Sensitivity: 50.00% | 7.375 | 0.006 | 1 |
| 5 healthy | 8.3% | (31.297%-68.703%) Specificity: 83.33% | ||||||
| 40 Negative | 15 Patient | 25% | (65.279%-94.358%) | |||||
| 25 healthy | 41.7% | Positive Predictive Value: 75.00% | ||||||
| (55.529%-87.816%) Negative | ||||||||
| Predictive Value: 62.50% (52.967%-71.153%) | ||||||||
| Disease prevalence: 50.00% (36.806%-63.194%) | ||||||||
| 30 samples Patient + 30 samples healthy people | Detection With dnaJ gene | 20 positive | 15 Patient | 25% | 7.375 | 0.006 | 1 | |
| 5 healthy | 8.3% | Sensitivity: 50.00% | ||||||
| (31.297%-68.703%) Specificity: 83.33% | ||||||||
| (65.279%-94.358%) | ||||||||
| 40 Negative | 15 Patient | 25% | Positive Predictive Value: 75.00% | |||||
| (55.529%-87.816%) Negative | ||||||||
| 25 healthy | 41.7% | Predictive Value: 62.50% (52.967%-71.153%) | ||||||
| Disease prevalence: 50.00% (36.806%-63.194%) |