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. 2021 Jan 20;17(1):e1009179. doi: 10.1371/journal.ppat.1009179

Fig 2. The LANA-FBW7 interaction specifically stabilizes the MCL-1 protein.

Fig 2

(A, upper left) After transfection with LANA-WT and/or FBW7, cells were treated with cycloheximide (CHX, 20 μg/ml) for the indicated periods, followed by IB with anti-Au, anti-Flag, or anti-β-actin antibodies. (A, bottom left) At 40 h post-transfection with LANA-P1 and/or FBW7, 293T cells were treated with CHX (20 μg/ml) for the indicated periods, followed by IB with the indicated antibodies. (A, upper and bottom right) Time course of expression was determined by semi-quantification of IB bands. (B) TREx/BJAB LANA/Au and TREx/BJAB LANA-P1/Au cells were treated with doxycycline (Doxy, 1 μg/ml) together with CHX (20 μg/ml) for the indicated periods. Equal amounts of total protein were analyzed by IB with the indicated antibodies. (C) Cells were transfected with the MCL-1 constructs together with the FBW7 and LANA or LANA-P1 constructs. Cells were then treated with CHX (20 μg/ml) for the indicated periods, followed by IB with the designated antibodies.