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. 2021 Jan 20;17(1):e1009179. doi: 10.1371/journal.ppat.1009179

Fig 5. KSHV-infection induces the MCL-1 stabilization in BJAB cells.

Fig 5

(A) (Left panel) BAC DNAs were digested with NheI restriction enzyme and subjected to PFGE analysis. (Right panel) BAC16 clones were confirmed by Sanger DNA sequencing. (B) WT, LANA-P1, Rev recombinant KSHV-infected BJAB were harvested and equal amounts of cell lysates were used for IB with the indicated antibodies. (C) BJAB-rKSHV-BAC16-WT, BJAB-rKSHV-BAC16-LANA-P1, and BJAB-rKSHV-BAC16-Rev cells were counted every 12 h. Error bars represent the SEM for three independent experiments. (D) After treatment with etoposide (50 μM), Cells were then stained with PI and carried out the FACS analysis. Data represent the mean ± SEM and P-values were calculated by Student’s t-test. NS, p > 0.05; *, p < 0.05; N = 3.