Skip to main content
. 2021 Jan 20;16(1):e0245169. doi: 10.1371/journal.pone.0245169

Fig 7. Inhibition of IL-10 synthesis or class 1 HDACs have variable effects on the secretion of chemotactic cytokines and chemokines.

Fig 7

Quantitative ELISA analysis of supernatants collected from murine alveolar macrophage cells (MH-S) cultures treated with DMSO, treated with DMSO and stimulated with 100 ng/mL LPS (DMSO + LPS), treated with 300 nM AS101 and stimulated with 100 ng/mL LPS (AS101 + LPS), treated with 10 μM MS275 and stimulated with 100 ng/mL LPS (MS275 + LPS), and treated with both 300 nM AS101 and 10 μM MS275 and treated with 100 ng/mL LPS (AS101 + MS275 + LPS). Precise quantities of A. CXCL2 (MIP2), B. IL6, and C. MIF were measured and compared between treatment groups. Data presented are representative of 3 independent experiments with 3 biological replica per group. A one-way ANOVA with Tukey’s correction was used to calculate differences between the groups * p ≤ 0.05, ** p ≤ 0.002, *** p ≤ 0.0002, **** p < 0.0001.