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. 2021 Jan 20;17(1):e1009219. doi: 10.1371/journal.ppat.1009219

Fig 4. HBZ overexpression alleviates Tax-induced senescence in vivo.

Fig 4

(A) SA-β-gal expression in larval eye-imaginal discs from control (GMR-GAL4>w1118), Tax Tg (GMR-Gal4>UAS-Tax), HBZ Tg (GMR-Gal4>UAS-HBZ) or Tax/HBZ Tg (GMR-Gal4; UAS-Tax>UAS-HBZ). (B) Levels of expression of Dacapo (p21/p27) in the transgenic adult flies heads as indicated. The values were normalized to Rp49. Reported values are the average of three independent experiments and error bars represent SD of triplicates p<0.01 (**) p<0.001 (***). (C) Levels of expression of Tax and HBZ transcript levels in control larvae (HMLΔ-Gal4>w1118), larvae expressing transgenic Tax alone (HMLΔ-Gal4>UAS-Tax), HBZ alone (HMLΔ-Gal4>UAS-HBZ) or both HBZ and Tax (HMLΔ-Gal4; UAS-Tax>UAS-HBZ). (D) SA-β-gal expression in circulating hemocytes in transgenic larvae as indicated. (E) Quantification of senescent hemocytes. Results are expressed as percentage of control. p<0.001 (***). (F) SA-β-gal expression in circulating hemocytes in larvae from the control mcherry RNAi (HMLΔ-Gal4> mCherry RNAi), control Tax/mCherry RNAi (HMLΔ-Gal4;UAS-Tax>mCherry RNAi), Tax/Relish RNAi (HMLΔ-Gal4;UAS Tax>Relish RNAi), Tax/E(z) RNAi (HMLΔ-Gal4;UAS-Tax>E(z) RNAi) and Tax/Suz12 RNAi (HMLΔ-Gal4;UAS-Tax>Suz12 RNAi) as indicated. Results are expressed as percentage of control. (ns = not significant), p<0.0001 (****).