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. 2021 Jan 20;17(1):e1009219. doi: 10.1371/journal.ppat.1009219

Fig 6. HBZ interacts with PRC2 components EZH2 and SUZ12 and is associated with H3K27me3 accumulation in human cells.

Fig 6

(A) HEK293T cells transiently transfected with Myc-HBZ. Western blot was performed with indicated antibodies. (B) HEK293T cells transiently transfected with Myc-HBZ. Myc immunoprecipitates (IP: Myc) were blotted against EZH2, SUZ12 and Tax (Left panel).–AB represents a control with no antibody. Corresponding control cell lysates are shown as indicated (Right panel). (C) HBZ-EZH2 and HBZ-SUZ-12 interactions detected by Duolink Proximity Ligation [73] assay in HeLa cells transfected with HBZ (Scale bar 10 μm), or MT-1 expressing endogenous HBZ. Nuclei were stained with 4,6 diamidino-2-phenylindole (DAPI) (blue). (Scale bar 5 μm). (D) Confocal microscopy (Z-Stacks) of HeLa cells transiently expressing Myc-HBZ, and showing partial co-localization of HBZ-EZH2 (Upper panel) and HBZ-SUZ12 (Lower panel). Nuclei were stained with 4,6 diamidino-2-phenylindole (DAPI) (blue) (Scale bars 10μm and 5μm respectively).