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. 2021 Jan 20;7(4):eabe1174. doi: 10.1126/sciadv.abe1174

Fig. 1. Tumor cells have greater glucose uptake but similar mitochondrial activity to T cells.

Fig. 1

(A and B) Representative plot (left) and tabulated data (right) for median fluorescence intensity (MFI) of glucose uptake (2-NBDG) (A) and mitochondrial activity (MitoTracker Deep Red) (B) of CD4+ T cells, CD8+ T cells, and EpCAM+CD45− tumor cells from ascites and tumor. (C) Proportion of CD4+ and CD8+ cells (of CD3+ T cells) within ascites and tumor. (D) Proportion of EpCAM+ (of CD45−) tumor cells within ascites and tumor. (E and F) Representative plot (left) and tabulated data (right) for glucose uptake (2-NBDG) (E) and mitochondrial activity (MitoTracker Deep Red) (F) of EpCAM+CD45− tumor and EpCAM−CD45− stromal cells from ascites and tumor. (G) Representative plots for CD25, CD137, and PD1 expression by flow cytometry. (H and I) CD25, CD137, and PD1 expression on CD4+ T cells (H) and CD8+ T cells (I). (J and K) Naïve, central memory (Tcm), effector (Teff), and effector memory (Tem) phenotype based on CCR7 and CD45RO expression. Representative plot (left) and tabulated data (right) for CD4+ T cells (J) and CD8+ T cells (K) from ascites and tumor. P values determined by paired t test (*P < 0.05, **P < 0.01, and ***P < 0.001). Lines indicate matched patients (n = 6). FMO, fluorescence minus one; MFI, median fluorescence intensity.