Table 1.
Quantification of ebony germ-line transmission by crossing nos>PE2 with pegRNA transgenes
| G0 cross | G1 parent sex | No. G1 crosses* | Founders, no. (%)† | Total G2 ebony progeny, no. (%) | ebony progeny with G111X edit, no. (%)‡ | Estimated% ebonyG111X progeny§ |
| nos>PE2 x pCFD3-PE-ebonyG111X (pegRNA only) | Male | 10 | 10/10 (100) | 118/1,117 (10.6) | 61/62 (98.4) | 10.6 |
| Female | 8 | 6/8 (75.0) | 135/849 (15.9) | 20/20 (100) | 15.9 | |
| nos>PE2 x pCFD5-PE3-ebonyG111X (sgRNA + pegRNA) | Male | 16 | 16/16 (100) | 982/2,278 (43.1) | 34/40 (85.0) | 36.6 |
| Female | 12 | 12/12 (100) | 382/1,665 (22.9) | 29/32 (90.6) | 20.7 | |
| nos>PE2 II x pCFD5-PE3-ebonyG111X (sgRNA + pegRNA) | Male | 9 | 9/9 (100) | 227/731 (31.1) | N.D. | N.D. |
| nos>PE2 III x pCFD5-PE3-ebonyG111X (sgRNA + pegRNA) | Male | 8 | 8/8 (100) | 243/732 (33.2) | N.D. | N.D. |
N.D., not determined.
*
Single male or female parents were crossed with w;;TM3,e1/TM6b,e1 and only those resulting in at least 100 progeny were counted.
†
Founders are defined as parents that produced at least one ebony progeny.
‡
Correct edit is defined as a 1-kb region flanking the target site that contains the G111X allele with no other indels or mutations.
§
Defined as the % ebony progeny x % ebony progeny with the G111X edit.