Fig. 2.

All three RAS receptors interact in transfected HEK-293T cells. A Sequential resonance energy transfer (SRET²) assays were performed in HEK-293T cells transfected with a constant amount of cDNA for MasR-Rluc (0.6 μg) or σ₁R-Rluc (0.3 μg) (as negative control), a constant amount of cDNA for AT₁R-GFP² (1 μg), and increasing amounts of cDNA for AT₂R-YFP (0.5 to 4.1 μg). Values are the mean ± SEM of 8 independent experiments performed in duplicate. B BRET assays were performed in HEK-293T cells transfected with a constant amount of cDNA for AT₂R-Rluc (0.5 μg), a constant amount of cDNA for AT₁R-YFP (3 μg), and increasing amounts of cDNA for MasR (0 to 1 μg). Values are the mean ± SEM of 5 independent experiments performed in triplicate. One-way ANOVA followed by Bonferroni’s multiple comparison post hoc tests were used for statistical analysis. C: Schematic representation of the SRET² assay. *p < 0.05, ***p < 0.0001 versus absence of MasR