Fig. 5.

AT₁-MasHet and AT₂-MasHet expression in microglial primary cultures treated with LPS and IFN-γ. A–E Expression of AT₁R-MasR and AT₂R-MasR heteromers in primary microglial cultures was determined by proximity ligation assay (PLA), which was performed using specific primary antibodies against AT₁, AT₂, and Mas receptors. Confocal microscopy images (stacks of 4 consecutive planes) show heteroreceptor complexes; nuclei are Hoechst-stained (blue). Microglial cultures were incubated for 48 h in the absence (A, C, D) or in the presence (B, E) of 1 μM LPS and 200 U/mL IFN-γ. Scale bar: 20 μm. F: Bar graph showing the percentage of AT₁R-MasR and AT₂R-MasR clusters as red dots/cell. **p < 0.01; Student’s t test versus resting cells