Fig. 7.

AT₂-MasHet functionality in primary cultures of microglia. A–D Microglia in primary cultures were incubated for 48 h with 1 μM LPS and 200 U/mL IFN-γ (C, D) or vehicle (A, B). Microglial cells were pretreated with selective receptor antagonists (300 nM candesartan for AT₁R, 1 μM PD123319 for AT₂R, and 500 nM A779 for MasR) and subsequently treated with selective agonists (100 nM Ang II for AT₁R, 300 nM CGP-42112A for AT₂R, and 250 nM Ang [1–7] for MasR). Cytosolic cAMP levels (A, C) and ERK1/2 phosphorylation (B, D) were subsequently determined. Values are the mean ± SEM of 5 independent experiments performed in triplicate. One-way ANOVA followed by Bonferroni’s multiple comparison post hoc test were used for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001 versus forskolin treatment in cAMP measurements (A, C) or versus vehicle in pERK assays (B, D)