Fig. 6.

UFMylation regulates IFN-γ– and LPS-dependent iNOS expression and activity through Ern1. (A and B) Average NO production and (C and D) average iNOS expression (geometric mean) as measured by flow cytometry in IFN-γ and LPS-stimulated WT-Cas9 and ΔUba5-Cas9 expressing control vector and sgRNAs targeting Ern1. Average data pooled from four to six independent experiments are represented as mean ± SEM relative to WT-Cas9 stimulated with 10 U IFN-γ/mL or 50 ng LPS/mL. *P < 0.05 determined by ANOVA with Tukey’s multiple comparison test; ns, not statistically significant.