Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Genetic reagent (Mus musculus) | C57BL/6J | Jackson Laboratories | Stock #: 000664 | |
Cell line (Homo sapiens) | 293-FT | Invitrogen | Cat #: R70007 | |
Antibody | PerCP/Cyanine5.5 rat monoclonal anti-human/mouse CD49f | BioLegend | Cat #: 313617 | 1:50 |
Antibody | Chicken polyclonal anti-GFP | Abcam | Cat #: ab13970 RRID:AB_300798 | IF 1:1000 |
Antibody | Mouse monoclonal anti-BrdU | Invitrogen | Cat #: MoBU-1 | IF: 1:100 |
Antibody | Mouse monoclonal anti-GAPDH | Proteintech | Cat #: 60004–1-Ig RRID:AB_2737588 | WB: 1:2000 |
Antibody | Peroxidase AffiniPure goat polyclonal Anti-Mouse IgG (H+L) | Jackson Immunoresearch | Cat #: 115-035-003 | WB: 1:5000 |
Antibody | Rabbit polyclonal anti-K10 | BioLegend | Cat #: Poly19054 | IF: 1:1000 |
Antibody | Rabbit polyclonal anti-Nore1A | Donated by G. Clark | Cat #: PAS17071 | WB: 1:1000 |
Antibody | Rabbit polyclonal anti-RFP | Rockland | Cat #: 6000-401-379 RRID:AB_11182807 | IF: 1:1000 |
Antibody | Rat monoclonal anti-Nidogen | Santa Cruz Biotechnology | Cat #: sc-33706 RRID:AB_627519 | IF: 1:1000 |
Commercial assay or kit | BCA Protein Assay Kit | Thermo Fisher | Cat #: 23225 |
|
Commercial assay or kit | Click-iT EdU cell proliferation kit for imaging, Alexa Fluor 555 | Thermo Fisher Scientific | Cat. #: C10337 |
|
Commercial assay or kit | DNeasy Blood and Tissue Kit | Qiagen | Cat #: 69504 | |
Commercial assay or kit | iScript Reverse Transcription Supermix | Bio-Rad | Cat #: 1708840 | |
Commercial assay or kit | Power SYBR Green PCR Master Mix | Thermo Fisher Scientific | Cat. #: 4367660 | |
Commercial assay or kit | SuperSignal West Femto Maximum Sensitivity Substrate | Thermo Fisher | Cat #: 34094 | |
Commercial assay or kit | Zero Blunt TOPO kit | Invitrogen | Cat #: 450245 |
|
Recombinant DNA reagent | FLAG-Nore1 | Addgene | Cat #: 1975 RRID:Addgene_1975 | |
Recombinant DNA reagent | tdTomato-C1 | Addgene | Cat #: 54653 RRID:Addgene_54653 |
|
Software, algorithm | Prism | GraphPad | RRID:SCR_002798 | |
Software, algorithm | Zen Black | Zeiss | RRID:SCR_018163 | |
Other | M.O.M. buffer | Vector Labs | Cat #: BMK-220 |