Fig. 3.

Liat1 self-associates and is targeted to the nucleolus. (A) Anti-Liat1 immunoblot of mouse testis cytosol fractionated by size exclusion chromatography. (B) BiFC between YN-Liat1 and Liat1-YC (Left) and between YN-Liat1 and YC-Liat1 (Right). YN indicates the N-terminal half of YFP; YC indicates the C-terminal half of YFP. (Scale bars: 10 µm.) (C) Colocalization of NPM1 and BiFC of YN-Liat1 and Liat1-YC. (Scale bar: 10 µm.) (D) A yeast two-hybrid assay was performed in S. cerevisiae by cotransforming Liat1-DBD and full-length Liat1-AD or the indicated Liat1 truncations fused to the AD. Interactions were detected as growth on SC medium lacking Leu, Trp, and His.