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. 2020 Dec 28;118(1):e2015887118. doi: 10.1073/pnas.2015887118

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Fig. 6. — Liat1 is modified by Jmjd6 hydroxylase activity. (A) Anti-Flag immunoblot of Jmjd6^3xflag or Jmjd6^N277A in the presence or absence of ^3xHALiat1. (B) Anti-HA immunoblot of ^3xHALiat1 in the cytosol and nuclear fractions in the absence or presence of increasing amounts of Jmjd6^3xflag (Upper). Anti-flag immunoblots indicated relative expression of Jmjd6^3xflag (Lower). (C) Anti-HA immunoblot of ^3xHALiat1 and ^3xHALiat1ΔK in the cytosol and nuclear fractions in the presence of wild-type Jmjd6 or Jmjd6^N277A (Upper). Anti-flag immunoblots indicated relative expression of Jmjd6^3xflag (Lower). IB: immunoblot using the indicated antibodies.