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. 2020 Dec 21;118(1):e2007526118. doi: 10.1073/pnas.2007526118

Fig. 2.

Fig. 2.

Branched actin cytoskeleton plays a role in p22-mediated cell-cell fusion, and p22 binds to ITSN-1. (A) Distribution of number of nuclei in p22-WT–expressing cells treated with cytoskeletal drugs from three independent transfections, mean number of each replicate, average from three independent transfections shown. P values are one-way ANOVA with Dunnett’s test where not significant (n.s.). P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. (B) Distribution of number of nuclei in p22-WT–expressing cells treated with control siRNA, siRNA targeting ARPC3 from three independent transfections, mean number of each replicate and average from three independent transfections shown. P values are two-way Student’s t test where **P < 0.01. (C) Diagram of predicted SH3 binding motif and predicted WH2 motif in p22 cytoplasmic tail. (D) Distribution of number of nuclei in p22-WT–, p22 WH2mut-, and p22 SH3mut-expressing cells from three independent transfections, mean number of each replicate and average from three independent transfections shown. P values are one-way ANOVA with Tukey’s test where ****P < 0.0001. (E) Western blot of coimmunoprecipitation of GFP-tagged p22-WT and p22-P149A with Intersectin-1.