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. 2020 Dec 21;118(1):e2007526118. doi: 10.1073/pnas.2007526118

Fig. 3.

Fig. 3.

Cdc42 and N-WASP signaling downstream of ITSN-1 is needed for p22-mediated cell-cell fusion. (A) Diagram of p22 binding to Intersectin-1 through a SH3 binding motif in its cytoplasmic tail. (B) In vitro binding assay with myc-tagged p22-WT as bait for purified GST-tagged SH3 domain A–E. (C) Distribution of number of nuclei in p22-WT–expressing cells treated with control siRNA or siRNA targeting ITSN-1 and/or overexpressing SH3A and SH3A-DHPH from three independent transfections, mean number of each replicate and average from three independent transfections shown. P values are two-way Student’s t test where *P < 0.05, ***P < 0.001. (D) Distribution of number of nuclei in p22-WT–expressing cells treated with drugs from three independent transfections, mean number of each replicate, average from three independent transfections shown. P values are one-way ANOVA with Dunnett’s test where not significant (n.s.) P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. (E) Distribution of number of nuclei N-WASP null mouse embryonic fibroblasts and control cells expressing p22-WT from three independent transfections, with mean number of each replicate, average from three independent transfections shown. P values are two-tailed Student’s t test where *P < 0.05.