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. 2020 Dec 21;118(1):e2007526118. doi: 10.1073/pnas.2007526118

Fig. 4.

Fig. 4.

p14 and p22 are modular cell-cell fusogens, and their cytoplasmic tails can be swapped. (A) Diagram of chimeric fusogen with p14 ectodomain, p14 transmembrane domain, and p22 cytoplasmic tail. (B) Confocal images of mCherry-tagged p22-WT, p14-WT, and p14/p22 chimera with plasma membrane labeled with CellMaskDeepRed (green). Boxed regions are magnified. (C) Average plasma membrane enrichment index of mCherry-tagged p22-WT and p14/p22 chimera from three independent transfections, and error bars represent SD 24 h posttransfection. P values are two-tailed Student’s t test where ****P < 0.0001. (D) Distribution of number of nuclei in p22-WT, p14-WT, and p14/p22 chimera expressing cells 24 h posttransfection from three independent transfections, with mean number of each replicate, average from three independent transfections shown. P values are one-way ANOVA with Tukey’s test where not significant (n.s.) P > 0.05, **P < 0.01. (E) Representative confocal image of p14/p22 chimera mCherry (magenta) cell with nuclei labeled with Hoechst 33342 (cyan) at 24 h posttransfection. (F) Mean GFP intensity in each cell expressing GFP-tagged p22-WT, p14-WT, and p14/p22 chimera 24 h posttransfection from three independent transfection and error bars represent SDs. P values are two-tailed Student’s t test where **P < 0.01, ***P < 0.001, ****P < 0.0001.