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. 2021 Jan 7;10:591698. doi: 10.3389/fonc.2020.591698

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Copyright © 2021 Guo, Zou, Zhou, Zhong, He, Huang, Chen, Li, Xiong, Fang and Xiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NUSAP1 interacts with YAP1. (A) The interaction between endogenous NUSAP1 and YAP1. The BGC823 cell lysates were immunoprecipitated with anti-YAP1 or control immunoglobulin G (IgG), followed by WB analysis with anti-NUSAP1 and anti-YAP1. (B) WB analysis of coprecipitating proteins in IPs performed using anti-Flag beads on lysates prepared from BGC823 cells. (C) WB analysis of coprecipitating proteins in IPs performed using anti-HA beads on lysates prepared from SGC7901 cells. (D) Immunofluorescence of NUSAP1 and YAP1 staining in BGC823 cells (magnification, ×400).