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. 2021 Jan 7;10:591698. doi: 10.3389/fonc.2020.591698

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Copyright © 2021 Guo, Zou, Zhou, Zhong, He, Huang, Chen, Li, Xiong, Fang and Xiang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NUSAP1 is required for the proliferation, migration, and invasion of GC cells in vitro. (A) HGC-27 and BGC823 cells were transfected with Flag-NUSAP1 or NUSAP1 shRNAs, and the efficiency was detected by Western blotting. (B, C) Representative images of colony-formation assays for modified HGC-27 and BGC823 cells. Cells were fixed and stained, the colonies were counted, and the data are represented in the bar graph. (D) Cell viability was analyzed by CCK-8 assay. (E, F) Representative images of fixed and stained modified HGC-27 and BGC823 cells in the Transwell invasion assays (magnification, ×200). (G, H) Cell migration ability evaluated by wound-healing assays (magnification, ×100). Student’s t test: **p<0.01.